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Associate Scientist III

Cambridge, MA, United StatesPosted 127 months ago
ContracthybridMid-Senior Level

Job Description

Specific Responsibilities Will Include: 

• Generation of reagents, such as CRISPR/Cas9 sgRNA, targeting vector, and bacterial artificial chromosome, to be used for genetic engineering in mice. 

• Validation of reagents using in vitro cleavage assay, PCR-based methods and DNA sequencing. 

Genotyping of models using PCR, qPCR, Southern blot and sequencing. 

• Independently review the relevant literature to understand and propose the best methods that can be applied to the needs of the group. 

• Interacting with peers and collaborators in a professional manner and productively. 

• Keep abreast of, and use, state-of-the-art technologies for generation of new GEMs. 

• Prepare, review and deliver scientific presentations for internal/external use. 


Qualifications include: 

• BS with at least 5 years or MS with at least three years of molecular biology experience in either academic or industry setting. 

• In depth knowledge and experience implementing molecular cloning techniques, particularly recombineering using bacterial artificial chromosomes. 

• Experience culturing and targeting embryonic stem cells. 

• Basic knowledge of mouse genetics. 

• Basic understanding genetic engineering in mice. 

• Experience using the nuclease technology, including CRISPR/Cas, TALEN and ZFN, will be given special consideration.


Warm Regards,

Jay Kaushik

Clinical Recruiter

Integrated Resources, Inc

IT REHAB CLINICAL NURSING

Inc. 5000 - 2007, 2008, 2009, 2010, 2011, 2012,2013 & 2014 (8th Year)

Certified MBE I GSA - Schedule 66 I GSA - Schedule 621I I GSA - Schedule 70

Direct: (732) 429 1633

Tel:(732) 549 2030x211

Fax: (732) 549 5549

http://www.irionline.com

 


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Associate Scientist III at Integrated Resources INC | Renata