Job Description
Mass General Brigham relies on a wide range of professionals, including doctors, nurses, business people, tech experts, researchers, and systems analysts to advance our mission. As a not-for-profit, we support patient care, research, teaching, and community service, striving to provide exceptional care. We believe that high-performing teams drive groundbreaking medical discoveries and invite all applicants to join us and experience what it means to be part of Mass General Brigham.
Job Summary
The Stanton Laboratory in the Center for Genomic Medicine (CGM) at Massachusetts General Hospital (MGH) is seeking a motivated, enthusiastic candidate to join our research group.Our research group works with brain 3D organoid model to understand the biological complexity, target identification, and validation in neurodegenerative disease. We are seeking a highly motivated Research Technician to join our research team focused on human induced pluripotent stem-cell (iPSC) models, gene editing, cellular phenotyping, and 3D brain organoid systems. The successful candidate will play a central role in supporting projects involving iPSC culture, CRISPR-based genome editing, directed differentiation, molecular cloning, and downstream validation assays.
This position is ideal for someone with strong hands-on experience in mammalian cell culture and molecular biology who is excited to contribute to a fast-paced, collaborative research environment. The technician will be expected to maintain rigorous experimental records, generate reproducible data, and assist in day-to-day laboratory operations.
Does this position require Patient Care? No
Perform laboratory experiments following established protocols and procedures.
Prepare and handle samples, reagents, and solutions, ensuring accuracy and adherence to safety guidelines.
Operate and maintain laboratory equipment and instruments, conducting routine calibration and troubleshooting as needed.
Record experimental data accurately and maintain organized records of procedures, protocols, and results.
Compile and analyze data using appropriate statistical and analytical methods.
Assist in data interpretation and presentation, contributing to research findings and reports.
Key responsibilities:
• Maintain and expand human iPSC lines under sterile culture conditions
• Conduct CRISPR-based gene editing workflows in iPSCs, including transfection/nucleofection, lentiviral transduction, clone handling, and expansion
• Support generation, validation, and maintenance of edited cell lines
• Perform directed differentiation of iPSCs into relevant downstream cell types
• Generate, culture, and maintain 3D brain organoids
• Assist with optimization and routine handling of organoid-based workflows
• Validate differentiated cells and organoid-derived cultures using - Flow cytometry, Immunofluorescence staining and imaging, and perform functional assay as required
• Carry out molecular cloning tasks such as plasmid preparation, bacterial transformation, restriction digest analysis, Gibson assembly, PCR, and construct verification
• Assist with genotyping, sequencing library preparation, and clone validation
• Maintain accurate, organized, and detailed lab notebooks and experimental records
• Contribute to protocol optimization, troubleshooting, and experimental planning
• Present data clearly in lab meetings and communicate progress to the research team
Prior experience below is a plus:
• Prior experience with human iPSC culture
• Experience with CRISPR/Cas9 gene editing
• Experience with stem cell differentiation
• Experience generating or maintaining brain organoids or other 3D culture systems
• Familiarity with aseptic technique, nucleofection, lentiviral production, and sequencing-based validation
• Previous experience in an academic, biotech, or translational research laboratory
Qualifications
Education
Bachelor's Degree Related Field of Study required
Can this role accept experience in lieu of a degree?
Yes
Licenses and Credentials
Experience
Prior laboratory experience, either through academic coursework, internships, or employment 1-2 years preferred
Knowledge, Skills and Abilities
- Strong theoretical and practical knowledge of laboratory techniques and procedures.
- Familiarity with common laboratory equipment and instruments used in research experiments.
- Proficiency in data collection, analysis, and scientific software tools.
- Excellent attention to detail, organizational skills, and the ability to multitask and prioritize tasks effectively.
- Strong problem-solving skills and the ability to troubleshoot technical issues.
- Excellent written and verbal communication skills to effectively convey experimental procedures, results, and challenges.
Additional Job Details (if applicable)
Remote Type
Work Location
Scheduled Weekly Hours
Employee Type
Work Shift
Pay Range
$21.00 - $29.01/Hourly
Grade
5
EEO Statement:
Mass General Brigham Competency Framework
At Mass General Brigham, our competency framework defines what effective leadership “looks like” by specifying which behaviors are most critical for successful performance at each job level. The framework is comprised of ten competencies (half People-Focused, half Performance-Focused) and are defined by observable and measurable skills and behaviors that contribute to workplace effectiveness and career success. These competencies are used to evaluate performance, make hiring decisions, identify development needs, mobilize employees across our system, and establish a strong talent pipeline.